Journal: Pathogens
Article Title: Novel Antigenic Variant Infectious Bursal Disease Virus Outbreaks in Japan from 2014 to 2023 and Characterization of an Isolate from Chicken
doi: 10.3390/pathogens13121141
Figure Lengend Snippet: Phylogenic tree of IBDV B2977CE2C3. A phylogenic tree based on the segment A sequence of the IBDV is shown. The IBDV B2977CE2C3 strain is shown in bold with a star. Relevant sequences of IBDV strains collected from GenBank are shown with the DNA database accession number. Seven genogroups, A1 to A7, of the IBDV strains classified using the Maximum Likelihood method are shown. Sub-genogroups (A2a, A2b, A2c, and A2d) further classified from genogroup A2 are also shown.
Article Snippet: If necessary, an amplified fragment was purified using the PCR cleaning kit (NucleoSpin Gel and PCR Clean-up Kit, Macherey-Nagel-Takara Bio Inc., Kusatsu, Japan) and was sequenced by ordering the sequencing service provider (Fasmac Co., Ltd., Atsugi, Japan).
Techniques: Sequencing